Psiadia punctulata (DC.) Vatke induces cell apoptosis in highly metastatic MDA-MB-231 cells

Purpose: This study assessed the in vitro cell migration inhibitory and apoptotic effects of P. punctulata stem (PPS (and leaf hexane) PPL (extracts on breast cancer lines (MDA-MB-231 andMCF-7 cells).Methods: Cytotoxicity was quantified using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) lactate dehydrogenase (LD) release test after 48 h exposure MDA-MB-231 cells to 0 – 200 μg/mL PPS hexane extract. Cell apoptosis determined Muse™ cytometry, while phytoconstituents extracts were identified by gas chromatography–mass spectrometry.Results: The half-maximal concentration (IC50) for against 44.33 52.16 μg/mL, respectively. T, whereas IC50 102.22 59.53 MCF-7 cells, sequentially. Treatment with 100 extract increased late 16.005 ± 1.155 52.58 3.02 %, respectively, 77.34 % 95.21 1.61 extract, when compared control (3.81% 0.79%). decreased filled ~15.5 wound gap 24 h, ~35 ~42.5 at contained several phytocompounds. Stem showed significant (p < 0.05) inhibition activities.Conclusion: exhibit potent cytotoxic activity tested cells. Further research is required assess acute subacute toxicity extracts.